Immuno-labelling, fluorescence microscopy and live time-lapse imaging Lab Report

Immuno-labelling, fluorescence microscopy and live time-lapse imaging - Lab Report Example The background of the alterations in the cell behaviour to include shape and movement is examined in the paper. The paper seeks to explain the significance of the microtubules in the cell, which is to increase the motility of the cells and the affinity of the cells on different reactions as detected in the body and the cell physiologies (Diaspro, 2011. p. 125). The examination of the process of immune labelling in the experiment to detect the presence and effects of the proteins in the cells is explained. The aims and objectives of an experiment basically answer the intent of the experiment. In this case the aims are directed towards the administration and examination of the cell physiology and the detection of the cell proteins and the reaction tests of the antibodies and antigens of the cell. This work explores on the background of the experiment and the purposes of the involvement of the cell physiological processes and the influences they have, such as the proteins, microtubules. The explanations of the experiment’s procedures, the tasks, actions, results and the findings are all covered in the paper. Introduction Immuno-labelling, fluorescence microscopy and live time-lapse imaging involves the cellular biological experiments which aim to detect as well as visualize the structures of the cells while analyzing the cellular changes in the cell and its substructures during a certain period of the body biological processes (Reimer & Kohl, 2008). This experiment involves the study of the immune system dynamics in the human tracheal cells. The involvement of the fluorescent microscopy in the experiment implies the detection of the fluorescent protein in the cell organelles, while observing other defects in the structure of the cell during the experiment. The experiment is not targeted at one result as the process includes diverse measures to analyze different structures in the cells. The process generally involves the analysis of the cell health to entire ty (Widdows, Kingdom & Ansari, 2009 pp 735). Immuno labelling is a biological process that involves the detection of antigen to a particular site in a cell, tissue or organ. In any immune labelling reaction the antigen and the antibody reacts within the given organ or tissue or cell in the experiment. The experimentation of the immuno labelling in the cell experiments are meant to reveal information about the cell or the substructures of the cells. In these processes, the antigens can be detected and visualizes using a combination of antigen-specific antibody in the process of detection referred to as tag, which is covalently related to the antibody (Tiwari & Nagai, 2013. pp 497). The immune cytochemistry, which is the process involving the imagery and analysis of the cell biology through fluorescent microscopy, compare the amplification strategies for the primary antibody detection in the cells. The results of the experiment after the detection analysis are used to direct action to wards achieving the recommended remedies (Hyatt & Wise, 2001. pp 97). Background of the Experimental Processes The cells of the immune system in the human body explore a wider existence territory than any other cells in the biological systems. The immune-imagery involves t6he visualization of the cells in the native tissues with the intent to detect the changes in the cell biological process of the cell. This process involves the quantification of the immune responses at the cellular level. It is through the process that the

Discipline and Punish by Michel Foucault Essay Example | Topics and Well Written Essays - 1500 words

Discipline and Punish by Michel Foucault - Essay Example Thesis: Power was the fulcrum around which all else revolved during the Classical period and through Foucault’s examples and arguments we understand how the body was debased through coercion and control, but gradually there came about a shift in power relations which we are experiencing today. In Foucault’s words - â€Å"Perhaps we should abandon the belief that power makes mad and that, by the same token, the renunciation of power is one of the conditions of knowledge. We should admit, rather, that power produces knowledge†¦.† (Michael Foucault, 1977, pp. 27/28) According to Foucault, both power and knowledge compliment each other and go hand in hand together. He explains that power relations cannot exist without the field of knowledge and in the same way, it is knowledge that contributes towards power relations. In Part I, Foucault speaks about torture that was used as a tool to discipline the body during the 18th century, giving us examples of torturous p enalties that were meted out during those days such as public executions. E.g. Damien’s torture (pgs. 3-5) This period of torture resulted in the ushering in of a new penal system for Europe and the United States. New theories involving law and crime were introduced while the ancient laws and customs were discarded and the new reforms were based on the political justification of punishment. According to Foucault, â€Å"justice no longer takes public responsibility for the violence that is bound up with its practice†¦Ã¢â‚¬  (Michael Foucault, 1977, p.9) However, with the introduction of the new penal system in our society today, judges have to judge something more than the crime and part of their powers are distributed to other authorities. â€Å"Today, criminal justice functions and justifies itself only by this perpetual reference to something other than itself, by this unceasing reinscription in non-juridical systems.   It’s fate is to be redefined by knowl edge† (22). In Part II, Foucault sheds light on ‘Punishment’. During this period punitive practices were redefined through refinement. Cold blooded criminality morphed into a criminality of fraud. This complex mechanism laid more emphasis and value on more stringent methods of surveillance and effective techniques of getting information which critics called ‘a bad economy of power.’ (79) The eighteenth century saw many reforms in the legal system such as new techniques and tactics for refining and regularizing the art of punishment and reducing the economic and political costs by making it more effective. In Part III, Foucault discusses about ‘Docile bodies’ that was directed towards coercion and supervising the activity rather than the result. In Foucault’s opinion, discipline serves to create docile bodies by disassociating the power from the body. On one hand, it increases an individuals capacity and aptitude but on the other ha nd, it arrests the flow of energy and brings about subjection. According to Foucault, â€Å"disciplinary coercion establishes in the body, the constricting link between an increased aptitude and an increased domination† (138) Among Foucault’s Disciplinary techniques, the one I have chosen for discussion is – ‘Control of Activity.’ Foucault describes discipline as a constant controlling of the activities,